Protein G antibody (HRP)

Catalog No. ABIN5596974

Supplier Product No.: 200-403-138-0100

The Rabbit Polyclonal anti-Protein G antibody has been validated for ELISA and DB. It is suitable to detect Protein G in samples from Streptococcus.

Quick Overview for Protein G antibody (HRP) (ABIN5596974)

Target: Protein G

Reactivity: Streptococcus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Protein G antibody is conjugated to HRP

Application: ELISA, Dot Blot (DB)

Product Details anti-Protein G Antibody (HRP)

Supplier Product No.: 200-403-138-0100

Supplier: Rockland

Purpose: Protein G Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].

Characteristics: Synonyms: rabbit anti-Protein G Antibody HRP Conjugation, Peroxidase conjugated rabbit anti-Protein G Antibody, Protein G HRP

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Protein G [Streptococcus species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Protein G Peroxidase Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Protein G

Target: Protein G

Background: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein G Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.