RORC antibody (pSer203)

Catalog No. ABIN5596977

Product Details anti-RORC Antibody

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Binding Specificity: pSer203

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity with this protein with pongo abelii based on 100% homology for the immunogen sequence.

Purification: Anti-nuclear receptor ROR gamma pS203 is directed against mouse receptor ROR gamma phosphorylated at the serine 203 position. This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.

Immunogen:

Immunogen: Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 61 kDa in size corresponding to receptor ROR gamma by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Alternative Name: Nuclear receptor ROR gamma (RORC Products)

Synonyms: NR1F3 antibody, RORG antibody, RZR-GAMMA antibody, RZRG antibody, TOR antibody, Nr1f3 antibody, RORgamma antibody, Thor antibody, RAR related orphan receptor C antibody, RAR-related orphan receptor C antibody, RAR-related orphan receptor gamma antibody, RORC antibody, Rorc antibody

Background:

Synonyms: Nr1f3, Rorg, Thor, RAR-related orphan receptor C, Nuclear receptor RZR-gamma, Thymus orphan receptor

Background: Anti-Nuclear receptor ROR gamma pS203 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nuclear receptor ROR gamma pS203 antibody detects mouse receptor ROR gamma phosphorylated at the serine 203 position. RAR-related orphan receptor gamma (ROR-γ) is a member of the nuclear receptor family of transcription factors. ROR-γ is a key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. ROR-γ regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. It is also involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism and is a negative regulator of adipocyte differentiation. It controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. Isoform 2, ROR-γt is essential for lymphoid organogenesis, in particular lymph nodes and Peyer's patches. ROR-γt also plays an important regulatory role in thymopoiesis, and in inhibiting apoptosis of undifferentiated T cells. Anti-nuclear receptor ROR gamma pS203 is ideal for researcher's interested in autoimmune diseases such as psoriasis and rheumatoid arthritis, circadian rhythms, and immune system disorders.

Gene Name: Rorc

Gene ID: 19885

NCBI Accession: NP_001280663

UniProt: P51450

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway