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RNASE1 antibody (HRP)

RNASE1 Reactivity: Cow WB, IP, ELISA Host: Rabbit Polyclonal HRP
Catalog No. ABIN5596978
  • Target See all RNASE1 Antibodies
    RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
    Reactivity
    • 33
    • 16
    • 15
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cow
    Host
    • 54
    • 3
    • 1
    Rabbit
    Clonality
    • 55
    • 3
    Polyclonal
    Conjugate
    • 25
    • 16
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RNASE1 antibody is conjugated to HRP
    Application
    • 51
    • 23
    • 18
    • 11
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Cross-Reactivity (Details)
    Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
    Purification
    Ribonuclease A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].
    Immunogen

    Immunogen: Ribonuclease A [Bovine Pancreas]

    Immunogen Type: Native Protein

    Isotype
    IgG
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    Discover our top product RNASE1 Primary Antibody
  • Application Notes

    Application Note: Anti-Ribonuclease A has been assayed against 1.0 μg of Ribonuclease A [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,500

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:10,000 - 1:40,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
    Alternative Name
    Ribonuclease A (RNASE1 Products)
    Synonyms
    RNASE1 antibody, RNS1 antibody, ATRNS1 antibody, RIBONUCLEASE 1 antibody, T17M13.16 antibody, T17M13_16 antibody, ribonuclease 1 antibody, RIB1 antibody, AI574248 antibody, Rib-1 antibody, Rib1 antibody, SRN antibody, ribonuclease A family member 1, pancreatic antibody, ribonuclease pancreatic-like antibody, ribonuclease A family member k6 antibody, Ribonuclease 1 antibody, ribonuclease 1 antibody, ribonuclease, RNase A family, 1 (pancreatic) antibody, ribonuclease pancreatic antibody, RNASE1 antibody, LOC475395 antibody, RNASE6 antibody, Rnase1 antibody, RNS1 antibody, LOC101113761 antibody
    Background

    Synonyms: Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

    Background: Ribonuclease A is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A cleaves specifically after pyrimidine nucleotides. It can be inhibited by ribonuclease inhibitor protein, by heavy metal ions, and by uridine-vanadate complexes. RNase A, and to a greater extent its oligomers and some homologs, have cytotoxic and cytostatic effects, particularly on cancer cells.

    Gene Name: RNASE1

    Gene ID
    282340
    UniProt
    P61823
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