APOB antibody

Catalog No. ABIN5596981

Supplier Product No.: 600-101-111

Product Details anti-APOB Antibody

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-101-111

Supplier: Rockland

Purpose: APOLIPOPROTEIN B Antibody

Cross-Reactivity (Details): Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.

Characteristics: Synonyms: goat anti-Apolipoprotein B antibody, Apo B-100, Apo B-48, APOB protein antibody, Apolipoprotein-B 100 antibody, Apolipoprotein B 48 antibody, Apolipoprotein B antibody, FLDB antibody

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Sterility: Sterile filtered

Immunogen:

Immunogen: apoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:500

Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References for anti-APOB antibody (ABIN5596981)

Morishita, Zhao, Tamura, Nishimura, Kanda, Sakamaki, Okazaki, Li, Mizushima: "A critical role of VMP1 in lipoprotein secretion." in: eLife, Vol. 8, (2020) (PubMed).

Sołtysik, Ohsaki, Tatematsu, Cheng, Fujimoto: "Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis." in: Nature communications, Vol. 10, Issue 1, pp. 473, (2019) (PubMed).

Lu, Yuan, Wang, Zhang, Yuan, Wu, Zhou, He, Lu, Chen, Fan, Liang, Cheng: "Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." in: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).

Nauli, Sun, Whittimore, Atyia, Krishnaswamy, Nauli: "Chylomicrons produced by Caco-2 cells contained ApoB-48 with diameter of 80-200 nm." in: Physiological reports, Vol. 2, Issue 6, (2014) (PubMed).

Boyer, Dumans, Beaumont, Etienne, Roingeard, Meunier: "The association of hepatitis C virus glycoproteins with apolipoproteins E and B early in assembly is conserved in lipoviral particles." in: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 18904-13, (2014) (PubMed).

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: APOB (APOB Products)

Background: Background: Anti Apolipoprotein B antibody recognizes the gene product of APOB that is the primary apolipoprotein of low-density lipoproteins, which is responsible for carrying cholesterol to tissues. While it is unclear exactly what functional role APOB plays in LDL, it is the primary apolipoprotein component and is absolutely required for its formation. What is clear is that the APOB on the LDL particle acts as a ligand for LDL receptors in various cells throughout the body. Through a mechanism that is not fully understood, high levels of APOB can lead to plaques that cause vascular disease (atherosclerosis), leading to heart disease. There is considerable evidence that levels of APOB are a better indicator of heart disease and cardiovascular risk than total cholesterol or LDL. However, primarily for historic reasons, cholesterol, and more specifically, LDL-cholesterol, remains the primary lipid test for the risk factor of atherosclerosis.

Gene ID: 338

UniProt: Q7Z7Q0

Pathways: Lipid Metabolism