PARP1 antibody (AA 1-100)

Catalog No. ABIN559798

This Mouse Monoclonal antibody specifically detects PARP1 in WB, ELISA and IF. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for PARP1 antibody (AA 1-100) (ABIN559798)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 3G4

Product Details anti-PARP1 Antibody

Binding Specificity: AA 1-100

Purpose: Mouse monoclonal antibody raised against a partial recombinant PARP1.

Sequence: MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PARP1 antibody (ABIN559798)

Faraoni, Compagnone, Lavorgna, Angelini, Cencioni, Piras, Panetta, Ottone, Dolci, Venditti, Graziani, Lo-Coco: "BRCA1, PARP1 and ?H2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib." in: Biochimica et biophysica acta, Vol. 1852, Issue 3, pp. 462-72, (2015) (PubMed).

Nicolae, Aho, Vlahos, Choe, De, Karras, Moldovan: "The ADP-ribosyltransferase PARP10/ARTD10 interacts with proliferating cell nuclear antigen (PCNA) and is required for DNA damage tolerance." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13627-37, (2014) (PubMed).

Shen, Oswald, Phelps, Cam, Pelloski, Pang, Houghton: "Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks." in: Cancer research, Vol. 73, Issue 11, pp. 3393-401, (2013) (PubMed).

Luijsterburg, Lindh, Acs, Vrouwe, Pines, van Attikum, Mullenders, Dantuma: "DDB2 promotes chromatin decondensation at UV-induced DNA damage." in: The Journal of cell biology, Vol. 197, Issue 2, pp. 267-81, (2012) (PubMed).

Pines, Vrouwe, Marteijn, Typas, Luijsterburg, Cansoy, Hensbergen, Deelder, de Groot, Matsumoto, Sugasawa, Thoma, Vermeulen, Vrieling, Mullenders: "PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1." in: The Journal of cell biology, Vol. 199, Issue 2, pp. 235-49, (2012) (PubMed).

Crawford, Yang, Mattaini, Hunter: "The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28660-73, (2009) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background: Full Gene Name: poly (ADP-ribose) polymerase 1
Synonyms: ADPRT,ADPRT1,PARP,PARP-1,PPOL,pADPRT-1

Gene ID: 142

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location