CHD4 antibody (AA 1632-1730)

Catalog No. ABIN560357

Product Details anti-CHD4 Antibody

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Binding Specificity: AA 1632-1730

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Mouse monoclonal antibody raised against a partial recombinant CHD4.

Sequence: ETEPKGAADV EKVEEKSAID LTPIVVEDKE EKKEEEEKKE VMLQNGETPK DLNDEKQKKN IKQRFMFNIA DGGFTELHSL WQNEERAATV TKKTYEIWH

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CHD4 (NP_001264, 1632 a.a. ~ 1730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 4H4

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CHD4 antibody (ABIN560357)

Guel, Scholtissek, Siegl, Fetter, Mayer, Bieber, Wenzel: "Dermatomyositis autoantigen CHD4 forms immune stimulatory complexes with endogenous DNA." in: Experimental dermatology, (2023) (PubMed).

Nishibuchi, Shibata, Hayakawa, Hayakawa, Ohtani, Sinmyozu, Tagami, Nakayama: "Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex." in: The Journal of biological chemistry, Vol. 289, Issue 42, pp. 28956-70, (2014) (PubMed).

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: CHD4 (CHD4 Products)

Background: Full Gene Name: chromodomain helicase DNA binding protein 4
Synonyms: DKFZp686E06161,Mi-2b,Mi2-BETA

Gene ID: 1108

NCBI Accession: NM_001273