GBA antibody (AA 146-235)

Catalog No. ABIN561009

Cited in 5+ publications. The Mouse Monoclonal anti-GBA antibody (Clone 2E2) (ABIN561009) specifically detects GBA in WB, ELISA, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GBA antibody (AA 146-235) (ABIN561009)

Target: GBA (Glucosidase, Beta, Acid (GBA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GBA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2E2

Product Details anti-GBA Antibody

Binding Specificity: AA 146-235

Purpose: Mouse monoclonal antibody raised against a partial recombinant GBA.

Sequence: SYFSEEGIGY NIIRVPMASC DFSIRTYTYA DTPDDFQLHN FSLPEEDTKL KIPLIHRALQ LAQRPVSLLA SPWTSPTWLK TNGAVNGKGS

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: GBA (NP_000148, 146 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-GBA antibody (ABIN561009)

Murphy, Gysbers, Abbott, Tayebi, Kim, Sidransky, Cooper, Garner, Halliday: "Reduced glucocerebrosidase is associated with increased ?-synuclein in sporadic Parkinson's disease." in: Brain : a journal of neurology, Vol. 137, Issue Pt 3, pp. 834-48, (2014) (PubMed).

Bendikov-Bar, Rapaport, Larisch, Horowitz: "Parkin-mediated ubiquitination of mutant glucocerebrosidase leads to competition with its substrates PARIS and ARTS." in: Orphanet journal of rare diseases, Vol. 9, pp. 86, (2014) (PubMed).

Bendikov-Bar, Horowitz: "Gaucher disease paradigm: from ERAD to comorbidity." in: Human mutation, Vol. 33, Issue 10, pp. 1398-407, (2012) (PubMed).

Bendikov-Bar, Ron, Filocamo, Horowitz: "Characterization of the ERAD process of the L444P mutant glucocerebrosidase variant." in: Blood cells, molecules & diseases, Vol. 46, Issue 1, pp. 4-10, (2011) (PubMed).

Campeau, Rafei, Boivin, Sun, Grabowski, Galipeau: "Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome." in: Blood, Vol. 114, Issue 15, pp. 3181-90, (2009) (PubMed).

Target Details for GBA

Target: GBA (Glucosidase, Beta, Acid (GBA))

Alternative Name: GBA

Background: Full Gene Name: glucosidase, beta, acid (includes glucosylceramidase)
Synonyms: GBA1,GCB,GLUC

Gene ID: 2629

NCBI Accession: NM_000157

Pathways: Cellular Glucan Metabolic Process