PRKAA2 antibody (AA 453-552)

Catalog No. ABIN5611223

This Mouse Monoclonal antibody specifically detects PRKAA2 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human and Monkey.

Quick Overview for PRKAA2 antibody (AA 453-552) (ABIN5611223)

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 8E11H5

Product Details anti-PRKAA2 Antibody

Binding Specificity: AA 453-552

Purpose: PRKAA2 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAA2

Background: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Molecular Weight: 62.3 kDa

Gene ID: 5563

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect