HNF4A antibody (AA 324-423)

Catalog No. ABIN561308

The Mouse Monoclonal anti-HNF4A antibody has been validated for WB, ELISA, IF and RNAi. It is suitable to detect HNF4A in samples from Human. There are 2+ publications available.

Quick Overview for HNF4A antibody (AA 324-423) (ABIN561308)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), RNA Interference (RNAi)

Clone: 4E2

Product Details anti-HNF4A Antibody

Binding Specificity: AA 324-423

Purpose: Mouse monoclonal antibody raised against a partial recombinant HNF4A.

Sequence: NDRQYDSRGR FGELLLLLPT LQSITWQMIE QIQFIKLFGM AKIDNLLQEM LLGGSPSDAP HAHHPLHPHL MQEHMGTNVI VANTMPTHLS NGQMCEWPRP

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: HNF4A (NP_000448, 324 a.a. ~ 423 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-HNF4A antibody (ABIN561308)

Collins, Hapke, Aravalli, Steer: "In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells." in: Hepatic medicine : evidence and research, Vol. 12, pp. 79-92, (2020) (PubMed).

Aravalli, Collins, Hapke, Crane, Steer: "Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells." in: Hepatic medicine : evidence and research, Vol. 12, pp. 15-27, (2020) (PubMed).

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background: Full Gene Name: hepatocyte nuclear factor 4, alpha
Synonyms: FLJ39654,HNF4,HNF4a7,HNF4a8,HNF4a9,MODY,MODY1,NR2A1,NR2A21,TCF,TCF14

Gene ID: 3172

NCBI Accession: NM_000457

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process