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SMAD3 antibody (AA 120-221)

Name: SMAD3 antibody (AA 120-221)
SKU: ABIN561715
Price: 716.57 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects SMAD3 in WB, ELISA, IHC (p) and PLA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

SMAD3 monoclonal antibody (M02), clone 7F3. (SMAD3 antibody (AA 120-221))

Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3. (SMAD3 antibody (AA 120-221))

Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. (SMAD3 antibody (AA 120-221))

ISt

Western Blot detection against Immunogen (36. (SMAD3 antibody (AA 120-221))

Detection limit for recombinant GST tagged SMAD3 is approximately 0. (SMAD3 antibody (AA 120-221))

ELISA

Proximity Ligation Analysis of protein-protein interactions between MAPK8 and SMAD3. (SMAD3 antibody (AA 120-221))

PLA

10 images

(1 reference)

Catalog No. ABIN561715

Quick Overview for SMAD3 antibody (AA 120-221) (ABIN561715)

Target

See all SMAD3 Antibodies

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This SMAD3 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Clone

7F3

Binding Specificity
40

34

27

27

25

12

11

11

8

6

6

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 120-221

Purpose

Mouse monoclonal antibody raised against a partial recombinant SMAD3.

Sequence

VCVNPYHYQR VETPVLPPVL VPRHTEIPAE FPPLDDYSHS IPENTNFPAG IEPQSNIPET PPPGYLSEDG ETSDHQMNHS MDAGSPNLSP NPMSPAHNNL DL

Cross-Reactivity

Human, Rat

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

SMAD3 (NP_005893, 120 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype

IgG1
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215) antibody
SMAD3 Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB24758 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
KO Validated SMAD3 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS, IF (cc) Host: Rabbit Monoclonal 3D1 unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Nagel, Ehrentraut, Tomasch, Quentmeier, Meyer, Kaufmann, Drexler, MacLeod: "Ectopic expression of homeobox gene NKX2-1 in diffuse large B-cell lymphoma is mediated by aberrant chromatin modifications." in: PLoS ONE, Vol. 8, Issue 4, pp. e61447, (2013) (PubMed).
Target

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name

SMAD3

Background

Full Gene Name: SMAD family member 3
Synonyms: DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396

Gene ID

4088

NCBI Accession

NM_005902

Pathways

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy