Topoisomerase II alpha antibody (AA 1435-1531)

Catalog No. ABIN563224

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Topoisomerase II alpha antibody (AA 1435-1531) (ABIN563224)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1E2

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1435-1531

Purpose: Mouse monoclonal antibody raised against a partial recombinant TOP2A.

Sequence: RAAPKGTKRD PALNSGVSQK PDPAKTKNRR KRKPSTSDDS DSNFEKIVSK AVTSKKSKGE SDDFHMDFDS AVAPRAKSVR AKKPIKYLEE SDEDDLF

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: TOP2A (NP_001058, 1435 a.a. ~ 1531 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Topoisomerase II alpha antibody (ABIN563224)

Poonperm, Takata, Hamano, Matsuda, Uchiyama, Hiraoka, Fukui: "Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins." in: Scientific reports, Vol. 5, pp. 11916, (2015) (PubMed).

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: Full Gene Name: topoisomerase (DNA) II alpha 170 kDa
Synonyms: TOP2,TP2A

Gene ID: 7153

NCBI Accession: NM_001067

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases