CD165 antibody

Catalog No. ABIN5633270

The Mouse Monoclonal anti-CD165 antibody (Clone SN2) (ABIN5633270) specifically detects CD165 in FACS, IP, WB, ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CD165 antibody (ABIN5633270)

Target: CD165 (CD165 Antigen (CD165))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD165 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: SN2

Product Details anti-CD165 Antibody

Purpose: Anti-Hu CD165 Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody SN2, also known as SN2 N6-D11, recognizes an extracellular epitope of CD165, an approximately 37-42 kDa transmembrane glycoprotein expressed mainly on leukemic T cells, double positive and double negative thymocytes (CD4-CD8-, CD4+CD8+), and platelets.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: CD165 purified from human Molt-4 cell line

Isotype: IgG1, kappa

Application Details

Application Notes: Immunohistochemistry (frozen sections): Application note: acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD165

Target: CD165 (CD165 Antigen (CD165))

Alternative Name: CD165

Background: CD165 Molecule,CD165 is a poorly characterized transmembrane protein highly expressed on platelets and many leukemic T cell lines. At lower level it is expressed on a proportion of circulating T cells and monocytes, on thymic epithelium, fibroblasts, epidermal keratinocytes, pancreatic islet cells, and some neurons. It might have a role in adhesion between thymocytes and thymic epithelial cells and it can be used as a marker for tumor progression.,AD2

Gene ID: 23449