IGF2R antibody (Biotin)

Catalog No. ABIN5633271

This Mouse Monoclonal antibody specifically detects IGF2R in WB, FACS and IP. It exhibits reactivity toward Human and Non-Human Primate.

Quick Overview for IGF2R antibody (Biotin) (ABIN5633271)

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF2R antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-238

Product Details anti-IGF2R Antibody (Biotin)

Purpose: Anti-Hu CD222 Biotin

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The antibody MEM-238 recognizes an extracellular epitope between amino acids 192-697 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10 % in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Recombinant Vaccinia virus encoding CD222.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Extracellular and intracellular staining, recommended dilution: 2-6 μg/mL.
Western blotting: Recommended dilution: 1 μg/mL, non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Alternative Name: CD222

Background: Insulin like growth factor 2 receptorprovided,CD222 (CIMPR, cation-independent mannose 6-phosphate receptor, IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10 % of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.,IGF2R, MPR1, CIMPR, MPR300, M6P-R

Gene ID: 3482

UniProt: P11717