UBTF antibody (AA 551-650)

Catalog No. ABIN563329

This Mouse Monoclonal antibody specifically detects UBTF in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for UBTF antibody (AA 551-650) (ABIN563329)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 6B6

Product Details anti-UBTF Antibody

Binding Specificity: AA 551-650

Purpose: Mouse monoclonal antibody raised against a partial recombinant UBTF.

Sequence: PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI GSRWQRISQS QKEHYKKLAE EQQKQYKVHL DLWVKSLSPQ DRAAYKEYIS

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-UBTF antibody (ABIN563329)

Fulka, Langerova: "The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition." in: Development (Cambridge, England), Vol. 141, Issue 8, pp. 1694-704, (2014) (PubMed).

Østrup, Strejcek, Petrovicova, Lucas-Hahn, Morovic, Lemme, Petersen, Laurincikova, Niemann, Laurincik, Hyttel: "Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle." in: Cellular reprogramming, Vol. 13, Issue 2, pp. 145-55, (2011) (PubMed).

Østrup, Petrovicova, Strejcek, Morovic, Lucas-Hahn, Lemme, Petersen, Niemann, Laurincik, Maddox-Hyttel: "Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryos." in: Cloning and stem cells, Vol. 11, Issue 3, pp. 367-75, (2009) (PubMed).

Song, Lee, Kim, Kim, Park, Kim, Chang, Han, Lee, Lee, Koo: "Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells." in: BMC developmental biology, Vol. 9, pp. 44, (2009) (PubMed).

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBTF

Background: Full Gene Name: upstream binding transcription factor, RNA polymerase I
Synonyms: NOR-90,UBF

Gene ID: 7343

NCBI Accession: NM_014233