SQSTM1 antibody (AA 1-440)

Catalog No. ABIN563860

This Mouse Monoclonal antibody specifically detects SQSTM1 in WB, ELISA and IF. It exhibits reactivity toward Human and has been mentioned in 88+ publications.

Quick Overview for SQSTM1 antibody (AA 1-440) (ABIN563860)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 2C11

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 1-440

Purpose: Mouse monoclonal antibody raised against a full length recombinant SQSTM1.

Sequence: MASLTVKAYL LGKEDAAREI RRFSFCCSPE PEAEAEAAAG PGPCERLLSR VAALFPALRP GGFQAHYRDE DGDLVAFSSD EELTMAMSYV KDDIFRIYIK EKKECRRDHR PPCAQEAPRN MVHPNVICDG CNGPVVGTRY KCSVCPDYDL CSVCEGKGLH RGHTKLAFPS PFGHLSEGFS HSRWLRKVKH GHFGWPGWEM GPPGNWSPRP PRAGEARPGP TAESASGPSE DPSVNFLKNV GESVAAALSP LGIEVDIDVE HGGKRSRLTP VSPESSSTEE KSSSQPSSCC SDPSKPGGNV EGATQSLAEQ MRKIALESEG RPEEQMESDN CSGGDDDWTH LSSKEVDPST GELQSLQMPE SEGPSSLDPS QEGPTGLKEA ALYPHLPPEA DPRLIESLSQ MLSMGFSDEG GWLTRLLQTK NYDIGAALDT IQYSKHPPPL

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SQSTM1 antibody (ABIN563860)

Korwitz, Merkwirth, Richter-Dennerlein, Tröder, Sprenger, Quirós, López-Otín, Rugarli, Langer: "Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria." in: The Journal of cell biology, Vol. 212, Issue 2, pp. 157-66, (2016) (PubMed).

Sydow, Hochgräfe, Könen, Cadinu, Matenia, Petrova, Joseph, Dennissen, Mandelkow: "Age-dependent neuroinflammation and cognitive decline in a novel Ala152Thr-Tau transgenic mouse model of PSP and AD." in: Acta neuropathologica communications, Vol. 4, pp. 17, (2016) (PubMed).

Winner, Beard, Hassiotis, Lau, Luck, Hopwood, Hemsley: "A Preclinical Study Evaluating AAVrh10-Based Gene Therapy for Sanfilippo Syndrome." in: Human gene therapy, Vol. 27, Issue 5, pp. 363-75, (2016) (PubMed).

He, Hamm, Reddy, Sams, Peliciari-Garcia, McGinnis, Bailey, Chow, Rowe, Chatham, Young: "Biotinylation: a novel posttranslational modification linking cell autonomous circadian clocks with metabolism." in: American journal of physiology. Heart and circulatory physiology, Vol. 310, Issue 11, pp. H1520-32, (2016) (PubMed).

Jin, Hong, Park, Chang, Hong, Park, Lee: "Inhibition of JNK-mediated autophagy enhances NSCLC cell sensitivity to mTORC1/2 inhibitors." in: Scientific reports, Vol. 6, pp. 28945, (2016) (PubMed).

Aydinlik, Erkisa, Cevatemre, Sarimahmut, Dere, Ari, Ulukaya: "Enhanced cytotoxic activity of doxorubicin through the inhibition of autophagy in triple negative breast cancer cell line." in: Biochimica et biophysica acta, Vol. 1861, Issue 2, pp. 49-57, (2016) (PubMed).

Redmann, Benavides, Berryhill, Wani, Ouyang, Johnson, Ravi, Barnes, Darley-Usmar, Zhang: "Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons." in: Redox biology, Vol. 11, pp. 73-81, (2016) (PubMed).

Garofalo, Iovine, Kuryk, Capasso, Hirvinen, Vitale, Yliperttula, Bevilacqua, Cerullo: "Oncolytic Adenovirus Loaded with L-carnosine as Novel Strategy to Enhance the Antitumor Activity." in: Molecular cancer therapeutics, Vol. 15, Issue 4, pp. 651-60, (2016) (PubMed).

Morgan, Thorburn: "Measuring Autophagy in the Context of Cancer." in: Advances in experimental medicine and biology, Vol. 899, pp. 121-43, (2016) (PubMed).

Wilson, Nikolic, Kentish, Shalini, Hatzinikolas, Page, Dorstyn, Kumar: "Sex-specific alterations in glucose homeostasis and metabolic parameters during ageing of caspase-2-deficient mice." in: Cell death discovery, Vol. 2, pp. 16009, (2016) (PubMed).

Conlon, Thomas, Fedotova, Hernandez-Ono, Di Paolo, Chan, Ruggles, Gibeley, Liu, Ginsberg: "Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis." in: The Journal of clinical investigation, Vol. 126, Issue 10, pp. 3852-3867, (2016) (PubMed).

de Luxán-Delgado, Caballero, Potes, Rubio-González, Rodríguez, Gutiérrez-Rodríguez, Solano, Coto-Montes: "Melatonin administration decreases adipogenesis in the liver of ob/ob mice through autophagy modulation." in: Journal of pineal research, Vol. 56, Issue 2, pp. 126-33, (2015) (PubMed).

Niso-Santano, Malik, Pietrocola, Bravo-San Pedro, Mariño, Cianfanelli, Ben-Younès, Troncoso, Markaki, Sica, Izzo, Chaba, Bauvy, Dupont, Kepp, Rockenfeller, Wolinski, Madeo, Lavandero, Codogno, Harper et al.: "Unsaturated fatty acids induce non-canonical autophagy. ..." in: The EMBO journal, (2015) (PubMed).

Kucukgul, Ozler, Inci, Karakas, Irmak, Gozuacik, Taralp, Koc: "3D bioprinting of biomimetic aortic vascular constructs with self-supporting cells." in: Biotechnology and bioengineering, Vol. 112, Issue 4, pp. 811-21, (2015) (PubMed).

Li, Shi, He, Hu, Wang, Zhang, Chan, Chen: "Streptococcus pneumoniae induces autophagy through the inhibition of the PI3K-I/Akt/mTOR pathway and ROS hypergeneration in A549 cells." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122753, (2015) (PubMed).

Porta, Kwong, Trojanowski, Lee: "Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases." in: Journal of neuropathology and experimental neurology, Vol. 74, Issue 4, pp. 380-7, (2015) (PubMed).

Yang, Peng, Ni, Li, Shi, Ding, Fan: "Basal Autophagy and Feedback Activation of Akt Are Associated with Resistance to Metformin-Induced Inhibition of Hepatic Tumor Cell Growth." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130953, (2015) (PubMed).

Dou, Xu, Donahue, Shimi, Pan, Zhu, Ivanov, Capell, Drake, Shah, Catanzaro, Ricketts, Lamark, Adam, Marmorstein, Zong, Johansen, Goldman, Adams, Berger: "Autophagy mediates degradation of nuclear lamina." in: Nature, Vol. 527, Issue 7576, pp. 105-9, (2015) (PubMed).

Cinque, Forrester, Bartolomeo, Svelto, Venditti, Montefusco, Polishchuk, Nusco, Rossi, Medina, Polishchuk, De Matteis, Settembre: "FGF signalling regulates bone growth through autophagy." in: Nature, Vol. 528, Issue 7581, pp. 272-5, (2015) (PubMed).

Tung, Wang, Hsu, Hu, Her, Huang, Liao: "Presenilin-1 regulates the expression of p62 to govern p62-dependent tau degradation." in: Molecular neurobiology, Vol. 49, Issue 1, pp. 10-27, (2014) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: Full Gene Name: sequestosome 1
Synonyms: A170,OSIL,PDB3,ZIP3,p60,p62,p62B

Gene ID: 8878

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy