G3BP1 antibody (AA 214-303)

Catalog No. ABIN564390

Cited in 4+ publications. The Mouse Monoclonal anti-G3BP1 antibody (Clone 2F3) (ABIN564390) specifically detects G3BP1 in WB, IF, ELISA, IHC (p) and RNAi. The antibody is reactive with Human samples.

Quick Overview for G3BP1 antibody (AA 214-303) (ABIN564390)

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 2F3

Product Details anti-G3BP1 Antibody

Binding Specificity: AA 214-303

Purpose: Mouse monoclonal antibody raised against a partial recombinant G3BP.

Sequence: KPEPVLEETA PEDAQKSSSP APADIAQTVQ EDLRTFSWAS VTSKNLPPSG AVPVTGIPPH VVKVPASQPR PESKPESQIP PQRPQRDQRV

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-G3BP1 antibody (ABIN564390)

Martin, Bellora, González-Vallinas, Irimia, Chebli, de Toledo, Raabe, Eyras, Urlaub, Blencowe, Tazi: "Preferential binding of a stable G3BP ribonucleoprotein complex to intron-retaining transcripts in mouse brain and modulation of their expression in the cerebellum." in: Journal of neurochemistry, Vol. 139, Issue 3, pp. 349-368, (2016) (PubMed).

Kaehler, Isensee, Hucho, Lehrach, Krobitsch: "5-Fluorouracil affects assembly of stress granules based on RNA incorporation." in: Nucleic acids research, Vol. 42, Issue 10, pp. 6436-47, (2014) (PubMed).

Martin, Zekri, Metz, Maurice, Chebli, Vignes, Tazi: "Deficiency of G3BP1, the stress granules assembly factor, results in abnormal synaptic plasticity and calcium homeostasis in neurons." in: Journal of neurochemistry, (2013) (PubMed).

Norazit, Meedeniya, Nguyen, Mackay-Sim: "Progressive loss of dopaminergic neurons induced by unilateral rotenone infusion into the medial forebrain bundle." in: Brain research, Vol. 1360, pp. 119-29, (2010) (PubMed).

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP1

Background: Full Gene Name: GTPase activating protein (SH3 domain) binding protein 1
Synonyms: G3BP,HDH-VIII,MGC111040

Gene ID: 10146

Pathways: SARS-CoV-2 Protein Interactome