Diazepam Binding Inhibitor antibody (AA 2-187)

Catalog No. ABIN5647032

This Rabbit Polyclonal antibody specifically detects Diazepam Binding Inhibitor in WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Diazepam Binding Inhibitor antibody (AA 2-187) (ABIN5647032)

Target: Diazepam Binding Inhibitor (DBI)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Diazepam Binding Inhibitor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Diazepam Binding Inhibitor Antibody

Binding Specificity: AA 2-187

Purification: Antigen affinity purified

Immunogen: A human recombinant protein corresponding to amino acids S2-I87 was used as the immunogen for the DBI antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DBI antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL,Flow cytometry: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the DBI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Diazepam Binding Inhibitor

Target: Diazepam Binding Inhibitor (DBI)

Alternative Name: DBI / Diazepam Binding Inhibitor

Background: Acyl-CoA-binding protein, also called Diazepam binding inhibitor, is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

UniProt: P07108