TREM1 antibody

Catalog No. ABIN5647283

This anti-TREM1 antibody is a Rabbit Polyclonal antibody detecting TREM1 in WB and ELISA (Capture). Suitable for Mouse and Rat.

Quick Overview for TREM1 antibody (ABIN5647283)

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA (Capture)

Product Details anti-TREM1 Antibody

Purification: Antigen affinity purified

Immunogen: A recombinant mouse protein corresponding to amino acids A21-S202 was used as the immunogen for the Trem1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Trem1 antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,ELISA (Capture, recombinant mouse protein): 0.1-0.5 μg/mL (BSA-free format available)

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Trem1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Alternative Name: Trem1

Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by TREM1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

UniProt: Q9JKE2