Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Characteristics
This antibody reacts to human LC3B (Autophagy-related protein LC3 B, Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.
Purification
Purified
Immunogen
A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.
MAP1LC3B
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry (FFPE): 1:100-1:200,Immunocytochemistry: 1:100-1:200,Western Blot: 1:100-1:200
Restrictions
For Research Use only
Buffer
Antibody in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant LC3B antibody at -20°C.
Target
LC3B (MAP1LC3B)
(Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
This antibody reacts to human LC3B (Autophagy-related protein LC3 B, Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.