Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Characteristics
This antibody reacts to human LC3B (Autophagy-related protein LC3 B, Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.
Purification
Purified
Immunogen
A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.
The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry (FFPE): 1:100-1:200,Immunocytochemistry: 1:100-1:200,Western Blot: 1:100-1:200
Restrictions
For Research Use only
Buffer
Antibody in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant LC3B antibody at -20°C.
Target
LC3B (MAP1LC3B)
(Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
This antibody reacts to human LC3B (Autophagy-related protein LC3 B, Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.