Mast Cell Tryptase antibody

Catalog No. ABIN5647716

This Rabbit Polyclonal antibody specifically detects Mast Cell Tryptase in IHC (p), WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Mast Cell Tryptase antibody (ABIN5647716)

Target: Mast Cell Tryptase

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mast Cell Tryptase antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Product Details anti-Mast Cell Tryptase Antibody

Purification: Antigen affinity purified

Immunogen: Amino acids H65-P275 from the human protein were used as the immunogen for the Mast Cell Tryptase antibody.

Isotype: IgG

Application Details

Application Notes: Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA: 0.1-0.5 μg/mL (human protein tested), request BSA-free format for coating

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: Prior to reconstitution, store at 4°C. After reconstitution, the Mast Cell Tryptase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Mast Cell Tryptase

Target: Mast Cell Tryptase

Background: Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

UniProt: Q15661