POLI antibody (AA 616-715)

Catalog No. ABIN564869

The Mouse Monoclonal anti-POLI antibody has been validated for WB and ELISA. It is suitable to detect POLI in samples from Human. There are 3+ publications available.

Quick Overview for POLI antibody (AA 616-715) (ABIN564869)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 8G9

Product Details anti-POLI Antibody

Binding Specificity: AA 616-715

Purpose: Mouse monoclonal antibody raised against a partial recombinant POLI.

Sequence: PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: POLI (AAH32662, 616 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-POLI antibody (ABIN564869)

Sekimoto, Oda, Kurashima, Hanaoka, Yamashita: "Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication." in: Molecular and cellular biology, Vol. 35, Issue 4, pp. 699-715, (2015) (PubMed).

Takezawa, Ishimi, Aiba, Yamada: "Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation." in: Journal of nucleic acids, Vol. 2010, pp. 750296, (2010) (PubMed).

Wang, Woodgate, McManus, Mead, McCormick, Maher: "Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations." in: Cancer research, Vol. 67, Issue 7, pp. 3018-26, (2007) (PubMed).

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: POLI

Background: Full Gene Name: polymerase (DNA directed) iota
Synonyms: RAD30B,RAD3OB

Gene ID: 11201

Pathways: DNA Damage Repair