SIRT1 antibody (AA 456-555)

Catalog No. ABIN565071

This anti-SIRT1 antibody is a Mouse Monoclonal antibody detecting SIRT1 in WB, ELISA, IF and IHC (p). Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SIRT1 antibody (AA 456-555) (ABIN565071)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7B7

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 456-555

Purpose: Mouse monoclonal antibody raised against a partial recombinant SIRT1.

Sequence: NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SIRT1 antibody (ABIN565071)

Kok, Lin, Hou, Hong, Chang, Hsiao, Wang, Lai, Lin: "Simvastatin inhibits cysteine-rich protein 61 expression in rheumatoid arthritis synovial fibroblasts through the regulation of sirtuin-1/FoxO3a signaling." in: Arthritis and rheumatism, Vol. 65, Issue 3, pp. 639-49, (2013) (PubMed).

Østrup, Hyttel, Klærke, Collas: "Remodeling of ribosomal genes in somatic cells by Xenopus egg extract." in: Biochemical and biophysical research communications, Vol. 412, Issue 3, pp. 487-93, (2011) (PubMed).

Hiraike, Wada-Hiraike, Nakagawa, Koyama, Miyamoto, Sone, Tanikawa, Tsuruga, Nagasaka, Matsumoto, Oda, Shoji, Fukuhara, Saji, Nakagawa, Kato, Yano, Taketani: "Identification of DBC1 as a transcriptional repressor for BRCA1." in: British journal of cancer, Vol. 102, Issue 6, pp. 1061-7, (2010) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: Full Gene Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Synonyms: SIR2L1

Gene ID: 23411

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling