RAD18 antibody (AA 332-430)

Catalog No. ABIN566046

Cited in 7+ publications. The Mouse Monoclonal anti-RAD18 antibody (Clone 3H7) (ABIN566046) specifically detects RAD18 in WB, ELISA, IF, IHC (p) and RNAi. The antibody is reactive with Human samples.

Quick Overview for RAD18 antibody (AA 332-430) (ABIN566046)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 3H7

Product Details anti-RAD18 Antibody

Binding Specificity: AA 332-430

Purpose: Mouse monoclonal antibody raised against a partial recombinant RAD18.

Sequence: EKEIDEIHSK YRKKHKSEFQ LLVDQARKGY KKIAGMSQKT VTITKEDEST EKLSSVCMGQ EDNMTSVTNH FSQSKLDSPE ELEPDREEDS SSCIDIQEV

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RAD18 antibody (ABIN566046)

Sy, Jiang, O, Deng, Huen: "The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks." in: Nucleic acids research, Vol. 41, Issue 18, pp. 8572-80, (2013) (PubMed).

Wong, Aguissa-Touré, Wani, Khosravi, Martinka, Martinka, Li: "Elevated expression of Rad18 regulates melanoma cell proliferation." in: Pigment cell & melanoma research, Vol. 25, Issue 2, pp. 213-8, (2012) (PubMed).

Kato, Waki, Umezawa, Aoki, Utsugi, Ohtsu, Murakami: "Phosphorylation of human INO80 is involved in DNA damage tolerance." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 433-8, (2012) (PubMed).

Wong, Lin, Khosravi, Piche, Jafarnejad, Chen, Li: "Tumour suppressor ING1b maintains genomic stability upon replication stress." in: Nucleic acids research, Vol. 39, Issue 9, pp. 3632-42, (2011) (PubMed).

Yanagihara, Kobayashi, Tateishi, Kato, Matsuura, Tauchi, Yamada, Takezawa, Sugasawa, Masutani, Hanaoka, Weemaes, Mori, Zou, Komatsu: "NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol ?-dependent translesion DNA synthesis." in: Molecular cell, Vol. 43, Issue 5, pp. 788-97, (2011) (PubMed).

Hicks, Chute, Paulsen, Ragland, Howlett, Guéranger, Glover, Canman: "Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links." in: Molecular and cellular biology, Vol. 30, Issue 5, pp. 1217-30, (2010) (PubMed).

Kobayashi, Okui, Asaithamby, Burma, Chen, Tanimoto, Matsuura, Komatsu, Chen: "WRN participates in translesion synthesis pathway through interaction with NBS1." in: Mechanisms of ageing and development, Vol. 131, Issue 6, pp. 436-44, (2010) (PubMed).

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18

Background: Full Gene Name: RAD18 homolog (S. cerevisiae)
Synonyms: RNF73

Gene ID: 56852

NCBI Accession: NM_020165