CD40 Ligand antibody (AA 105-260)

Catalog No. ABIN5662007

This Rabbit Monoclonal antibody specifically detects CD40 Ligand in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for CD40 Ligand antibody (AA 105-260) (ABIN5662007)

Target: CD40 Ligand (CD40LG)

Reactivity: Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C4

Product Details anti-CD40 Ligand Antibody

Binding Specificity: AA 105-260

Purpose: Monoclonal Antibody to Cluster Of Differentiation 40 Ligand (CD40L)

Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - KKEKSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVVLENGRQL TVKREGLYYV YTQVTFCSNR EPLSQRPFIV SLWLKPSSGS ERILLRAANT HSSSKLCEQQ SIHLGGVFEL QAGASVFVNV TEASQVIHGI GFSSIGLLKL

Specificity: The antibody is a mouse monoclonal antibody raised against CD40L. It has been selected for its ability to recognize CD40L in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cluster Of Differentiation 40 Ligand (CD40L) corresdonding to Lys105~Leu260 (Accession # Q9Z2V2) with N-terminal His and GST Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L

Background: CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints