DOT1L antibody (AA 3-108)

Catalog No. ABIN566567

There is 1 publication for this product available. The Mouse Monoclonal anti-DOT1L antibody is suitable to detect DOT1L in samples from Human. It has been validated for WB, ELISA and IF.

Quick Overview for DOT1L antibody (AA 3-108) (ABIN566567)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 6A6

Product Details anti-DOT1L Antibody

Binding Specificity: AA 3-108

Purpose: Mouse monoclonal antibody raised against a partial recombinant DOT1L.

Sequence: EKLELRLKSP VGAEPAVYPW PLPVYDKHHD AAHEIIETIR WVCEEIPDLK LAMENYVLID YDTKSFESMQ RLCDKYNRAI DSIHQLWKGT TQPMKLNTRP STGLLR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-DOT1L antibody (ABIN566567)

Phillips, Wildt, Comizzoli: "Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis." in: Journal of assisted reproduction and genetics, Vol. 33, Issue 6, pp. 783-94, (2016) (PubMed).

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background: Full Gene Name: DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Synonyms: DKFZp586P1823,DOT1,KIAA1814,KMT4

Gene ID: 84444

NCBI Accession: NM_032482