Recombinant Flavivirus Group Antigen antibody

Catalog No. ABIN5668107

Product Details

Target: Flavivirus Group Antigen

Antibody Type: Recombinant Antibody

Reactivity: Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Yellow Fever Virus

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Specificity: Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

Characteristics:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Purification: Purified antibody.

Immunogen: Dengue Virus type 2 antigens.

Clone: D1-4G2-4-15 (4G2)

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

References

Xu, Shan, Dunn, Xie, Xia, Gao, Allende Labastida, Zou, Villarreal, Schlagal, Yu, Vargas, Rossi, Vasilakis, Shi, Weaver, Wu: "Role of microglia in the dissemination of Zika virus from mother to fetal brain." in: PLoS neglected tropical diseases, Vol. 14, Issue 7, pp. e0008413, (2020) (PubMed).

Forster, Schwarz, Brosinski, Kalinke, Sutter, Volz: "Obstetric Ultrasonography to Detect Fetal Abnormalities in a Mouse Model for Zika Virus Infection." in: Viruses, Vol. 12, Issue 1, (2020) (PubMed).

Vermillion, Lei, Shabi, Baxter, Crilly, McLane, Griffin, Pekosz, Klein, Burd: "Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes." in: Nature communications, Vol. 8, pp. 14575, (2018) (PubMed).

Target Details

Target: Flavivirus Group Antigen

Target Type: Viral Protein

Background: Protein E, Envelope Protein