Recombinant CD19 antibody

Catalog No. ABIN5668223

This Rabbit Chimeric antibody specifically detects CD19 in FACS, IHC, IP, IF, BP and SPR. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD19 antibody (ABIN5668223)

Target: CD19 (CD19 Molecule (CD19))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Peptide (BP), Surface Plasmon Resonance (SPR)

Clone: HD37

Product Details anti-CD19 Antibody

Purpose: Anti-CD19 [HD37], Rabbit IgG, kappa

Specificity: This antibody is specific for human CD19, a 95 kDa protein expressed in the B-lymphocyte lineage.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Cells from a hairy cell leukemia patient.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to CD19 and is able to inhibit B-cell activation (Pezutto 1987). This antibody was measured to have a K_D of 4.1 ± 0.3 nM against human CD19 and did not compete with clone FMC63 (available as Ab00613) (Sakemura et al., 2023, PMID: 37879074).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: Leu12, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19 T-cell surface antigen Leu-12, CD19

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway