CHRNB3 antibody (N-Term)

Catalog No. ABIN5671353

This Rabbit Polyclonal antibody specifically detects CHRNB3 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Guinea Pig, Cow, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for CHRNB3 antibody (N-Term) (ABIN5671353)

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNB3 Antibody

Binding Specificity: N-Term

Purpose: CHRNB3 Antibody - N-terminal region

Sequence: QGYQKWVRPV LHSNDTIKVY FGLKISQLVD VDEKNQLMTT NVWLKQEWTD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ACHB3. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ACHB3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34971-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHRNB3

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Alternative Name: CHRNB3

Background:

Background Information: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]

Gene Name: cholinergic receptor nicotinic beta 3 subunit

Protein Name: neuronal acetylcholine receptor subunit beta-3

Molecular Weight: 50kDa

Gene ID: 1142

NCBI Accession: NP_000740

UniProt: Q05901