EIF3B antibody (N-Term)

Catalog No. ABIN5672523

This anti-EIF3B antibody is a Rabbit Polyclonal antibody detecting EIF3B in WB. Suitable for Human.

Quick Overview for EIF3B antibody (N-Term) (ABIN5672523)

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3B Antibody

Binding Specificity: N-Term

Sequence: AAEELPGSHA EPPVPAQGEA PGEQARDERS DSRAQAVSED AGGNEGRAAE

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: EIF3B

Background: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

Alias Symbols: PRT1, EIF3S9, EIF3-ETA, EIF3-P110, EIF3-P116

Protein Size: 873

Gene ID: 8662

NCBI Accession: NM_001037283, NP_001032360

Pathways: Ribonucleoprotein Complex Subunit Organization