Cyclin E1 antibody

Catalog No. ABIN567784

This Mouse Monoclonal antibody specifically detects Cyclin E1 in IF and IP. It exhibits reactivity toward Human.

Quick Overview for Cyclin E1 antibody (ABIN567784)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: HE172

Product Details anti-Cyclin E1 Antibody

Specificity: This antibody reacts with Cyclin E (50 kDa) on Imunoprecipitation.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Human recombinant full length His-Cyclin E.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background: Cyclin E, a positive regulator of the cell cycle, controls the transition of cells from G1 to S phase. Cyclin E forms a complex with Cdk2, the active kinase follows cyclin D/Cdk4 in progressively phosphorylating pRb, releasing it from members of the E2F family. E2F transcrIPPtion factors in turn activate a set of target genes that are essential for entry into S phase. Deregulation of the G1-S checkpoint contributes to uncontrolled cell division, a hallmark of cancer. Cyclin E overexpression and deregulation have been shown in several cancers including breast, colorectal, gastric and lung.Synonyms: CCNE, CCNE1, Cyclin-E1

Gene ID: 898

NCBI Accession: NP_001229

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases