Fibronectin antibody

Catalog No. ABIN5680165

The Rabbit Polyclonal anti-Fibronectin antibody has been validated for WB, IF, ELISA, IHC (p) and RIA. It is suitable to detect Fibronectin in samples from Rat.

Quick Overview for Fibronectin antibody (ABIN5680165)

Target: Fibronectin

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Specificity: Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution)

Cross-Reactivity (Details): Rat

Characteristics: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution).

Purification: Affinity purified

Immunogen: Purified fibronectin from rat plasma

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Rat skin,IHC(P), IFA, ELISA, RIA, IB (WB),IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Preservative: Without preservative

Storage: -20 °C

Target Details for Fibronectin

Target: Fibronectin