COL6 antibody

Catalog No. ABIN5680257

This Rabbit Polyclonal antibody specifically detects COL6 in WB, ELISA, IF, IHC (p) and RIA. It exhibits reactivity toward Human.

Quick Overview for COL6 antibody (ABIN5680257)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-COL6 Antibody

Specificity: Human collagen type VI 100 % binding in RIA at 1:10.000 dilution, cross-reactivity with human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % Human, cross-reactive with bovine

Cross-Reactivity: Chicken, Cow (Bovine), Mouse (Murine), Pig (Porcine), Rat (Rattus)

Cross-Reactivity (Details): Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)

Characteristics: Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100 % , human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % (RIA at 1:10.000 dilution).

Purification: Affinity purified

Immunogen: Purified collagen type VI from human placenta

Isotype: IgG

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human placenta,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Alternative Name: Collagen Type VI