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Alanine Dehydrogenase antibody

The Rabbit Polyclonal anti-Alanine Dehydrogenase antibody is suitable to detect Alanine Dehydrogenase in samples from Bacillus. It has been validated for DB, EIA, ID, IF, IP, RIA and WB.
Catalog No. ABIN568055
$546.00
Plus shipping costs $50.00
10 mg
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Quick Overview for Alanine Dehydrogenase antibody (ABIN568055)

Target

Alanine Dehydrogenase

Reactivity

Bacillus

Host

  • 1
Rabbit

Clonality

  • 1
Polyclonal

Conjugate

  • 1
This Alanine Dehydrogenase antibody is un-conjugated

Application

Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
  • Specificity

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes L-Alanine Dehydrogenase.

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

    Immunogen

    L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis. Freund's complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL of sterile distilled water.

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins.

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Alanine Dehydrogenase

    Background

    This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.Synonyms: BSU31930, Stage V sporulation protein N, ald, spoVN

    Gene ID

    936557

    NCBI Accession

    NP_391071

    UniProt

    Q08352
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