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Alanine Dehydrogenase antibody (Biotin)

Reactivity: Bacillus DB, EIA, ID, IF, IP, RIA, WB Host: Rabbit Polyclonal Biotin
Catalog No. ABIN568056
  • Target See all Alanine Dehydrogenase products
    Alanine Dehydrogenase
    Reactivity
    Bacillus
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This Alanine Dehydrogenase antibody is conjugated to Biotin
    Application
    Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
    Specificity
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes L-Alanine Dehydrogenase.
    Characteristics
    Molar Ratio: ~5.4
    Purification
    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
    Immunogen
    L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1.0 mL of sterile distilled water.
    Concentration
    10.0 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives and foreign proteins.
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target
    Alanine Dehydrogenase
    Abstract
    Alanine Dehydrogenase Products
    Synonyms
    BA0592 antibody, BA4873 antibody, alanine dehydrogenase antibody, ald1 antibody, ald2 antibody, ald antibody, Mesil_3009 antibody, Trad_2499 antibody
    Background
    This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.Synonyms: BSU31930, Stage V sporulation protein N, ald, spoVN
    Gene ID
    936557
    NCBI Accession
    NP_391071
    UniProt
    Q08352
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