Alcohol Oxidase antibody
Reactivity: Candida sp.
IF, WB, EIA, ID, IP, RIA, DB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Alcohol Oxidase
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Reactivity
- Candida sp.
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Host
- Rabbit
-
Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
- Immunogen
- Alcohol dehydrogenase isolated and purified from Candida boidinii. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore by adding 1.0 mL of sterile distilled water, Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
- Concentration
- 10.0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins.
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C. Reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- Alcohol Oxidase
- Background
- Alcohol Oxidase (AOX) is a homooctameric flavoprotein consisting of eight identical subunits of ~74 kD, each containing a flavin adenine dinucleotide molecule (FAD) as a prosthetic group (van der Klei et al., 1991). The protein catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of certain yeasts including Pichia pastoris, Hansenula polymorpha, and Candida boidinii (van der Klei et al., 1991). AOX is normally localized in the matrix of single membrane-bound organelles called peroxisomes. During methanol growth, the peroxisomes also contain large amounts of dihydroxyacetone synthase, the first enzyme in the methanol assimilatory pathway, and catalase (CAT), which converts the hydrogen peroxide generated by oxidases such as AOX into water and oxygen (Veenhuis and Harder, 1991). As a result, peroxisomes, which are small and few in number in glucose-grown cells, are massively induced in methanol-grown cells (Veenhuis and Harder, 1991).Synonyms: AOD1, AOX, MOX, Methanol Oxidase
- Gene ID
- 5477
- UniProt
- Q00922
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