Ceruloplasmin antibody

Catalog No. ABIN568138

This Rabbit Polyclonal antibody specifically detects Ceruloplasmin in WB, IP, IHC (p), IF, EIA, RIA, ID and DB. It exhibits reactivity toward Human.

Quick Overview for Ceruloplasmin antibody (ABIN568138)

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ceruloplasmin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-Ceruloplasmin Antibody

Specificity: The antibody recognizes Ceruloplasmin from Human serum. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), Block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Ceruloplasmin isolated and purified from Human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL of sterile distilled water. Prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) and preferably use the same day.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ceruloplasmin

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Alternative Name: Ceruloplasmin

Background: Caeruloplasmin is a copper binding plasma glycoprotein consisting of a single polypeptide chain. It has a molecular weight of 132 kDa and occurs in plasma at a concentration of 150-500 mg/L. Although the physiological functions of Caeruloplasmin are not fully understood it is implicated in a number of possible functions. 95 % of plasma copper is bound to Caeruloplasmin, implying some involvement in copper transport. Caeruloplasmin has been described as both an oxidant and an antioxidant, its exact role or roles in this respect are not clear. Plasma levels of Caeruloplasmin are elevated after inflammation and trauma and it is therefore classified as an acute phase protein. The measurement of Caeruloplasmin in plasma is useful in the diagnosis of Wilson's disease, where levels are greatly reduced. Caeruloplasmin is extremely labile in plasma and rapidly degrades to 115 kDa and 19 kDa fragments. The age of plasma samples should therefore be taken into account when interpreting immunological analysis of caeruloplasmin.Synonyms: CP, Ferroxidase

Gene ID: 1356

NCBI Accession: NP_000087

UniProt: P00450

Pathways: Transition Metal Ion Homeostasis