This Rabbit Polyclonal antibody specifically detects Choline Kinase alpha in WB, IF, IP, EIA, DB, ID and RIA. It exhibits reactivity toward Saccharomyces cerevisiae.
Catalog No. ABIN568147
Quick Overview for Choline Kinase alpha antibody (ABIN568147)
The antibody recognizes Choline kinase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Choline kinase isolated and purified from Baker's Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
CHKA
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.