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Choline Kinase alpha antibody

This Rabbit Polyclonal antibody specifically detects Choline Kinase alpha in WB, IF, IP, EIA, DB, ID and RIA. It exhibits reactivity toward Saccharomyces cerevisiae.
Catalog No. ABIN568147

Quick Overview for Choline Kinase alpha antibody (ABIN568147)

Target

See all Choline Kinase alpha (CHKA) Antibodies
Choline Kinase alpha (CHKA)

Reactivity

  • 32
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  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Saccharomyces cerevisiae

Host

  • 35
  • 3
Rabbit

Clonality

  • 38
Polyclonal

Conjugate

  • 20
  • 7
  • 4
  • 3
  • 2
  • 2
This Choline Kinase alpha antibody is un-conjugated

Application

  • 33
  • 26
  • 8
  • 6
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
  • Specificity

    The antibody recognizes Choline kinase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Choline kinase isolated and purified from Baker's Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL of sterile distilled water

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Choline Kinase alpha (CHKA)

    Alternative Name

    Choline Kinase

    Background

    Synonyms: CKI, CKI1

    Gene ID

    850824

    NCBI Accession

    NP_013234

    UniProt

    P20485
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