The Biotin-conjugated Rabbit Polyclonal anti-alpha-Chymotrypsin antibody (ABIN568158) specifically detects alpha-Chymotrypsin in IF, WB, DB, EIA, ID, IP and RIA.
The antibody is reactive with Cow samples.
Quick Overview for alpha-Chymotrypsin antibody (Biotin) (ABIN568158)
Target
alpha-Chymotrypsin
Reactivity
Cow
Host
Rabbit
Clonality
Polyclonal
Conjugate
Biotin
Application
Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Characteristics
Molar Ratio: Biotin/IgG ~6.5
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Alpha-Chymotrypsinogen A isolated and purified from Bovine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.