GLO1 antibody

Catalog No. ABIN568243

This Rabbit Polyclonal antibody specifically detects GLO1 in WB, IF, IP, EIA, RIA, DB and ID. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for GLO1 antibody (ABIN568243)

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)

Product Details anti-GLO1 Antibody

Specificity: The antibody recognizes Glyoxalase from Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Solid Phase Affinity Chromatography

Immunogen: Glyoxalase isolated and purified from Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL of sterile distilled water

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: Glyoxalase I / GLO1

Background: The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.Synonyms: Aldoketomutase, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase

Gene ID: 855009

NCBI Accession: NP_013710

UniProt: P50107