Hexokinase 1 antibody (Biotin)

Catalog No. ABIN568253

The Rabbit Polyclonal anti-Hexokinase 1 antibody has been validated for WB, IP, IF, EIA, RIA, ID and DB. It is suitable to detect Hexokinase 1 in samples from Saccharomyces cerevisiae.

Quick Overview for Hexokinase 1 antibody (Biotin) (ABIN568253)

Target: Hexokinase 1 (HK1)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-Hexokinase 1 Antibody (Biotin)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.

Characteristics: Molar Ratio: Biotin/IgG = ~5.4

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Hexokinase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: Hexokinase-1

Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 Molecules/cell in log phase SD medium.Synonyms: HKA, HXK1, Hexokinase PI, Hexokinase-A

Gene ID: 850614

NCBI Accession: NP_116711

UniProt: P04806

Pathways: Carbohydrate Homeostasis, Warburg Effect