HAase antibody (Biotin)

Catalog No. ABIN568256

The Rabbit Polyclonal anti-HAase antibody is suitable to detect HAase in samples from Sheep. It has been validated for WB, IP, IF, DB, EIA, ID and RIA.

Quick Overview for HAase antibody (Biotin) (ABIN568256)

Target: HAase (Hyaluronidase (HAase))

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-HAase Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG: ~1.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Hyaluronidase isolated and purified from Sheep testes. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for HAase

Target: HAase (Hyaluronidase (HAase))

Alternative Name: Hyaluronidase

Background: Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis.Synonyms: HYAL-1, HYAL1, HYAL2, Hyal-2, Hyaluronidase, Hyaluronoglucosaminidase, LUCA-1, LUCA1

Pathways: Glycosaminoglycan Metabolic Process