Lactate Dehydrogenase (2H2M) (LDH-3) antibody (Biotin)

Catalog No. ABIN568288

Product Details

Target: Lactate Dehydrogenase (2H2M) (LDH-3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)

Characteristics: Molar Ratio: Biotin/IgG = ~5.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: L-Lactic dehydrogenase, LDH-3(H2M2) isoenzyme, is isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water.

Concentration: 10.0 mg/m

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details

Target: Lactate Dehydrogenase (2H2M) (LDH-3)

Alternative Name: LDH3 H2M2 Isozyme

Background: Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-3 Isozyme H2M2