Glucose-6-Phosphate Isomerase (PGI1) antibody

Catalog No. ABIN568369

This Rabbit Polyclonal antibody specifically detects Glucose-6-Phosphate Isomerase (PGI1) in DB, EIA, ID, IF, IP, RIA and WB. It exhibits reactivity toward Saccharomyces cerevisiae. It has been mentioned in 4+ publications

Quick Overview for Glucose-6-Phosphate Isomerase (PGI1) antibody (ABIN568369)

Target: Glucose-6-Phosphate Isomerase (PGI1)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)

Product Details

Specificity: IgG fraction of polyclonal rabbit antiserum to Phosphoglucose Isomerase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Phosphoglucose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

References

Cáceres-Gorriti, Carmona, Barrès, Rahimi, Létourneau, Tonin, Provencher, Mes-Masson: "RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 are new prognostic biomarkers in serous epithelial ovarian cancer." in: PLoS ONE, Vol. 9, Issue 3, pp. e91000, (2016) (PubMed).

Miura, Ueda: "Evaluation of unconventional protein secretion in Saccharomyces cerevisiae." in: Methods in molecular biology (Clifton, N.J.), Vol. 1270, pp. 51-70, (2015) (PubMed).

Miettinen, Björklund: "Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions." in: Nucleic acids research, Vol. 43, Issue 2, pp. 1019-34, (2015) (PubMed).

Ryu, Kwak, Lee, Kang, Oh, Lee, Kim, Ma, Kim: "Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells." in: Toxicology and applied pharmacology, Vol. 255, Issue 1, pp. 94-102, (2011) (PubMed).

Target Details

Target: Glucose-6-Phosphate Isomerase (PGI1)

Background: Synonyms: NLK, Neuroleukin, PGI1, PHI, Phosphoglucose isomerase, Phosphohexose isomerase

Gene ID: 852495

NCBI Accession: NP_009755

UniProt: P12709