Biotin-conjugated IgG fraction of polyclonal sheep antiserum to phosphoglyceric mutase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Characteristics
Molar Ratio: Biotin/IgG ~6.6
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phosphoglyceric mutase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.