PLA2G1B antibody (Biotin)

Catalog No. ABIN568378

This Rabbit Polyclonal antibody specifically detects PLA2G1B in WB, IF, ID, IP, DB, EIA and RIA. It exhibits reactivity toward Bee.

Quick Overview for PLA2G1B antibody (Biotin) (ABIN568378)

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Reactivity: Bee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G1B antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunodiffusion (ID), Immunoprecipitation (IP), Dot Blot (DB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Product Details anti-PLA2G1B Antibody (Biotin)

Specificity: Phospholipase A2 from bee venom. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), Block Titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~6.1

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Phospholipase A2 isolated and purified from bee venom. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid Repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for PLA2G1B

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Alternative Name: Phospholipase A2

Background: Phospholipase A2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, a precursor of eicosanoids including prostaglandins and leukotrienes. This enzyme has been proposed to hydrolyze phosphatidylcholine in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci. Phospholipase A2 IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of Phospholipase A2 IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.Synonyms: Allergen Api m I, Phosphatidylcholine 2-acylhydrolase

Gene ID: 406141

NCBI Accession: NP_001011614

UniProt: P00630

Pathways: Inositol Metabolic Process, VEGF Signaling