MPI antibody (Biotin)

Catalog No. ABIN568395

The Biotin-conjugated Rabbit Polyclonal anti-MPI antibody (ABIN568395) specifically detects MPI in WB, IF, IP, ID, EIA, RIA and DB. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for MPI antibody (Biotin) (ABIN568395)

Target: MPI (Mannose Phosphate Isomerase (MPI))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPI antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)

Product Details anti-MPI Antibody (Biotin)

Specificity: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phosphomannose isomerase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Characteristics: Molar Ratio: Biotin/IgG ~5.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Phosphomannose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for MPI

Target: MPI (Mannose Phosphate Isomerase (MPI))

Alternative Name: MPI

Background: Synonyms: Mannose-6-phosphate isomerase, PMI1, Phosphohexomutase, Phosphomannose isomerase

Gene ID: 856720

NCBI Accession: NP_010918

UniProt: P29952