PHKA1 antibody (Biotin)

Catalog No. ABIN568398

The Biotin-conjugated Sheep Polyclonal anti-PHKA1 antibody (ABIN568398) specifically detects PHKA1 in WB, IF, DB, EIA, ID, IP and RIA. The antibody is reactive with Rabbit samples.

Quick Overview for PHKA1 antibody (Biotin) (ABIN568398)

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This PHKA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Product Details anti-PHKA1 Antibody (Biotin)

Specificity: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to phosphorylase a from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Characteristics: Molar Ratio: Biotin/ IgG ~6.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromat

Immunogen: Phosphorylase a isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for PHKA1

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Alternative Name: PHKA1 / PHKA

Background: Synonyms: Phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform, Phosphorylase kinase alpha M subunit

Gene ID: 100303771

NCBI Accession: NP_001159389

UniProt: P18688

Pathways: Cellular Glucan Metabolic Process