psi-D Subunit of Photosystem I antibody

Catalog No. ABIN5684022

Cited in 3+ publications. The Rabbit Polyclonal anti-psi-D Subunit of Photosystem I antibody (ABIN5684022) specifically detects psi-D Subunit of Photosystem I in CN-PAGE, IP and WB. The antibody is reactive with Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomytrium Patens, Picea glauca, Pinus strobus, Spinach, Synechocystis, Triticosecale, Triticum aestivum and Zea mays samples.

Quick Overview for psi-D Subunit of Photosystem I antibody (ABIN5684022)

Target: psi-D Subunit of Photosystem I

Reactivity: Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomytrium Patens, Picea glauca, Pinus strobus, Spinach, Synechocystis, Triticosecale, Triticum aestivum, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Clear Native PAGE (CN-PAGE), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Purpose: Anti-PsaD | PSI-D subunit of photosystem I

Cross-Reactivity (Details): Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays
Not reactive in: Synechococcus elongatus sp. PCC 7942

Predicted Reactivity: Alge,Dicots,Catalpa bungei,Cucumis melo,Conifers,Cyanidioschyzon merolae,Bigelowiella natans,Nannochloropsis sp.,Phaeodactylum tricornutum,Phyla dulcis,Zosteria marina

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana PSI-D1 UniProt:Q9S7H1

Application Details

Application Notes: 1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)

Comment:

this antibody is a replacement for former product, anti-PsaD AS04 046

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, (2013) (PubMed).

Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Target Details

Target: psi-D Subunit of Photosystem I

Alternative Name: PSI-D subunit of photosystem I (PsaD)

Background:

Background: PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

Additional information: PsaD has frequently been used as a marker for intact PSI reaction centers.This product can be sold containing proclin if requested.

Molecular Weight: 17.9 | 20 (for Arabidopsis thaliana)

Gene ID: 838400

UniProt: Q9SA56, Q9S7H1