Ficin antibody

Catalog No. ABIN568455

Product Details anti-Ficin Antibody

Target: Ficin

Reactivity: Fig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ficin antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Ficin

Target: Ficin

Abstract: Ficin Products

Background: Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)